From the Biological ESTEEM collection

ESTEEM Category

Biochemistry

Description

This workbook calculates the necessary components to create a buffer solution. The workbook will allow the user to create a buffer in two ways. A user can select an acid and its conjugate base or select an acid or base and combine it with a strong base or strong acid. Users will input the desired pH, concentration and volume of the solution and the workbook will output a recipe for a buffer solution using the Henderson-Hasselbalch equation. Note that this workbook requires that "macros" are enabled in Microsoft Excel.

Go to the Buffer Preparation module in a new window

Primary References

Henderson, L.J. (1908) Concerning the relationship between the strength of acids and their capacity to preserve neutrality. American Journal of Physiology. Volume 21: 173-179.

Henderson, L.J. (1908) THe theory of neutrality regulation in the animal organism. American Journal of Physiology. Volume 21: 427-448. Hasselbalch, K.A. (1916) Biochemistry. Z., 78: 112-144.

Good, N.E.; Wingset, G.D.; Winter, W.; Connolly, T.N.; Izawa, S.; Singh, R.M.M. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry, 5:467-477.

Jack, R. Cecil. (1995) Basic Biochemical Laboratory Procedures and Computing. Oxford University Press, New York.

Popular Text Citations

Moore, J.W., C.L. Stanitski and P.C. Jurs, (2002). Chemistry The Molecular Science. Harcourt College Publishers, Orlando

Harris, D.C., (1987). Quantitative Chemical Analysis. W.H. Freeman and Company, New York. Second Edition.

Education Research & Pedagogical Materials

"Chemistry and Background" (2003)  ChemLab Darthmouth College. Trustees of Darthmouth College.

Po, H.N. and Senozan, H.M. (2001) The Henderson-Hasselbalch Equation: Its History and Limitations. Journal of Chemical Education, 78: 1499-1503.