From the Biological ESTEEM collection

ESTEEM Category

Bioinformatics

Description

This workbook allows the analysis of sample or imported protein sequences. The model can analyze protein sequences up to 500 amino acids long. The program analyzes five aspects of the protein sequence: the highest potential charge along the protein sequence, the amino acid composition of the protein sequence, the isoelectric point of the protein sequence at varying pHs, the hydrophobicity to predict surface and membrane spanning regions of the protein sequence and the protein structure using the Chau and Fassman algorithm. Note that this workbook requires that "macros" are enabled in Microsoft Excel.

Go to the Protein Analysis module in a new window

Primary References

Kyte J and Doolittle RF: A simple method for displaying the hydropathic character of a protien. J Mol Biol 157:105, 1982.

Shaw, G., (1998). 'Spreadsheets in Molecular Biology'. In Spreadsheets in Science and Engineering. Springer-Verlag, Berlin, Pages 203-228.

Shaw, G., (1995). Protein sequence interpretation using a spreadsheet program. BioTechniques, Volume 19, Pages 978-983.

Popular Text Citations

“Protein Hydrophobicity Plots”. (1998) Colorado State University. http://arbl.cvmbs.colostate.edu/molkit/hydropathy/index.html (Accessed 30 June 2005)

White, S. (2002) “Experimentally determined hydrophobicity scales”. From The Stephen White Laboratory at UC Irvine. http://blanco.biomol.uci.edu/hydrophobicity_scales.html (Accessed 1 July 2005)

Current Research Articles

Jungck, J.R. (1978) The Genetic Code as a Periodic Table. Journal of Molecular Evolution, 11: 211-224.

Hopp, T.P.; Woods, K.R. (1981) Prediction of protein antigenic determinants from amino acid sequences. Immunology, 78:3824-3828.